PCR and mutagenisis of the digestion sites
PCR1a: replication of Pdu D with mutagenisis
PCR1b: replication of Pdu D with mutagenisis
PCR2: Joining PCR of PduD
PCR3a: replication of Pdu C with mutagenisis
PCR3b: replication of Pdu C with mutagenisis
PCR4: Joining PCR of Pdu C
PCR5: replication of AurF to be joined with Pdu D
PCR6a: replication of LctO with mutagenisis
PCR6b: replication of LctO with mutagenisis
PCR7: Joining PCR of LctO
PCR8a: replication of LctO with mutagenisis
PCR8b: replication of LctO with mutagenisis
PCR9: Joining PCR of LctO
PCR10: Joining PCR of AurF and PduD
PCR11: Joining PCR of LctO and PduC
PCR12a: replication of the Pdu-Operon with mutagenisis
PCR12b: replication of the Pdu-Operon with mutagenisis
PCR13: Joining PCR of the Pdu-Operon
PCR14a: replication of the Pdu-Operon with mutagenisis
PCR14b: replication of the Pdu-Operon with mutagenisis
PCR15: Joining PCR of the Pdu-Operon
PCR16a: replication of the Pdu-Operon with mutagenisis
PCR16b: replication of the Pdu-Operon with mutagenisis
PCR17: Joining PCR of the Pdu-Operon
In Vitro testing of the chemical kinetics with the enzymes
Transforming the construct in bacteria
Testing the system
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