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Monday 12.07.2010

• OD measurement of Asaia’s culture
• Chemical competence for E.Coli DB3.1 (step : Day 3)
• PCR (Asaia ORI + primers)
• Made GLY agar plates without antibiotics -> failed (medium was too old)
• Asaia O/N culture without antibiotics in order to recover some WT
• Text for the sponsor

Tuesday 13.07.2010

• Chemical competence for E.Coli DB3.1 (step : Day 4) -> stored at -80°C
• Preparation of GLY Medium / LB Medium / SOC Medium / TAE 1X / TAE 0.5X
• Run a gel for the PCR -> failed (mix of two different kits)
• Preparation of plates (GLY Agar / LB+Kan Agar / LB+Amp Agar)
• Learned to autoclave
• Restarted PCR (Asaia ORI + primers)
• Plated Asaia (O/N culture)
• Choice of Antibiotics Biobricks resistance (Kan / Amp / Tet / Chl) from the iGem Kit and preparation of them (streak out)
• Transformation of E.coli DB3.1 with pUC19 to check its competence
• Transformation of E.coli DH5 with p1003 (Kan) / p1002 (Amp) / p1004 (Chl) / p1005 (Tet)
• E.coli DB3.1 and DH5 were plated on LB+Amp Agar plates (Kan was plated on LB+Kan Agar plate)
• Ordered material
• Wiki brainstorming
• Protocols printing and organization