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From 2010.igem.org

Chair: Da, Minutes: Younus, Comp

1. . Cloning Strategies

• SR1 - Finished
• RocF not started - need to use Gibson method;
• YneA
• 2 for Swarming (sfp and swrA)
• LacI
• 2 for Subtilin (Immunity and Production)

Only finished one, SR1. 7 more to go!

Next: swarming and subtilin nearly finished, YneA nearly coming back and LacI brick not sure how to test. Why is testing LacI hard? YneA - our most important BB - Phil, Rach, Deena Swarming - Harsh, Alan, Da LacI (2) - Phil, Younus Subtilin (2) - Harsh, Steve, Da RocF - Harsh, Alan, Steve,

5 parts to a Cloning Strategy

1. Purpose and justification
2. Modelling
3. Construction
4. Cloning and Integration
5. Testing and Characterising

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