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Contents 1 Bacillus sphaericus Rehydration 1.1 Aims 1.2 Materials 1.3 Procedure

Bacillus sphaericus Rehydration

Aims

We aimed to rehydrate and plate the sphaericus strain bought from BCCM. We used nutrient media to rehydrate the cells. The cells are going to be used to test the strength of Levans glue.

Materials

1. Nutrient Broth
2. Nutrient Plates
3. Pasteur pipette

Procedure

Freeze- dried cultures are supplied in vacuum- sealed ampoules.

1. Make a small file mark on the ampoule enar the middle of the cotton wool plug.
2. Touch the file mark with a red hot glass rod till the glass cracks.
3. Wait a few seconds to allow air ot enter into the amploule.
4. Remove the upper part of the ampoule and the cotton plug.
5. Add approximately 0.5ml of nutrient broth with a sterile Pasteur pipette to the dried material after flaming the open end of the ampoule.