Team:Wisconsin-Madison/parts

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Revision as of 21:50, 23 October 2010 by Msagstetter (Talk | contribs)

New Standards

Wisconsin-Madison Team has taken the initiative to improve maintenance of the Registry of Standard Parts with more user experience in the form of zip files! Each fill will be labeled by its corresponding part number in the registry (ie. BBa_K318501) and will contain the following:

  • Genbank including features and plasmid (.gbk???)
  • Fwd and Reverse sequencing files (.ab1)
  • Gels from cloning and confirmation (.pdf)
  • All associated testing files and Results (.xls, .pdf, etc.)

Enzyme Delivery

New DNA

Enzyme Delivery Testing Constructs
Part Number About Testing in... Zip File
<partinfo>BBa_k318512</partinfo> Regulatory - pH inducible promoter <partinfo>BBa_k318513</partinfo> 512
<partinfo>BBa_k318514</partinfo> Gene - RamA protein can bind to cholic acid (bile salts) <partinfo>BBa_k318515</partinfo> File:Zip file ramA.zip
<partinfo>BBa_k318516</partinfo> Regulatory - RamA/Bile-salt conjugate inducible promoter <partinfo>BBa_k318517</partinfo> 516


Testing Constructs

Enzyme Delivery Testing Constructs
Part Number Function Expression Type Zip File
<partinfo>BBa_k318500</partinfo> Produces Trascription Factor RcsB Inducible - IPTG 500
<partinfo>BBa_k318501</partinfo> Produces Trascription Factor RcsA Inducible - IPTG 501
<partinfo>BBa_k318502</partinfo> Produces Trascription Factor RcsA & RcsB Inducible - IPTG 502
<partinfo>BBa_k318503</partinfo> Produces Trascription Factor YgiV - Blocks Biofilm Inducible - IPTG 503
<partinfo>BBa_k318504</partinfo> Produces RfaI gene - WaaI Ligase Inducible - IPTG 504
<partinfo>BBa_k318505</partinfo> Produces T.F. YgiV & RfaI gene Inducible - IPTG 505
<partinfo>BBa_k318506</partinfo> Produces RFP (+)LuxR, (-)cILVA 506
<partinfo>BBa_k318509</partinfo> Produces Repressor cI-LVA (degredation tag) Inducible - IPTG 509
<partinfo>BBa_k318510</partinfo> Produces Repressor cI (no degredation tag) Inducible - IPTG 510
<partinfo>BBa_k318511</partinfo> Produces Activator LuxR Anhydrous Tetrycycline 511
<partinfo>BBa_k318513</partinfo> Produces RFP pH 513
<partinfo>BBa_k318515</partinfo> Produces Trascription Factor RcsA & RcsB RamA/Bile-Salt Complex 515
<partinfo>BBa_k318517</partinfo> Produces RamA gene Bile Salt 517


Final Constructs

Enzyme Delivery Final Constructs
Part Number Function Expression Type
<partinfo>BBa_k3185##</partinfo> Produces Trascription Factors RcsA & RcsB, and B-Gal Constitutive
<partinfo>BBa_k3185##</partinfo> A) Produces equivalent ratio of Activator(LuxR) and Repressor(cI-LVA) pH 2
<partinfo>BBa_k318507</partinfo> A) Activates Lysis once in the small intestine (+)-LuxR, (-)-cILVA
<partinfo>BBa_k318519</partinfo> B) Activates lysis once in the small intestine Bile Salts


Encryption

Encryption Constructs
Part Number Description Notes Zip File
<partinfo>BBa_K318000</partinfo> Key Cassette - produces Cre and Hin recombinases Arabinose induces Cre expression, IPTG induces Hin expression File:BBa K318000 pSB1C3.zip
<partinfo>BBa_K318030</partinfo> Lock Cassette - prevents transcription unless "unlocked" Requires successive Hin and Cre recombination for constitutive expression of downstream gene(s) File:BBa K318030 pSB1C3.zip


Other Parts

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K318010 BBa_K318010]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K318011 BBa_K318011]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K318012 BBa_K318012]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K318013 BBa_K318013]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K318014 BBa_K318014]