Overview

Our projects aims to help stopping the propagation of malaria. To do so, we chose to act on the mosquito. Indeed, if we prevent the mosquito from being infected by malaria, it won't be able to transmit the parasite to healthy humans. Our idea is to engineer asaia, a bacteria that is naturally present in the mosquito's intestinal tract. This bacteria would express an immunotoxin to kill the plasmodium and thus prevent the mosquito infection.

Movie

Coming soon!

Plan

After choosing our bacteria of interest, here Asaia, we decided on two main set of experiments.

• Cloning

The first of course, was the cloning part. We performed all the cloning in E.coli, and did experiments on Asaia transformation, growth and resistance to antibiotics in parallel. Here is the parts we successfully cloned. As E.Coli origin does not work in Asaia, we added an origin that was compatible with this bacteria. Gwen: where did we get this origin from? On the opposite, Asaia's origin does work in E.Coli, which is very useful for our sets of experiments.

• dessin des "parts"*

• Drosophila

In parallel, we did experiments to assess if Asaia was persistant in Drosophila. In fact,