Team:Newcastle/26 July 2010

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Contents

Colony PCR of Genomic DNA

Aim:

To determine whether the genes have been inserted into the plasmid of B. Subtilis 168.

Materials:

  • Pipette
  • Microfuge
  • Microtubes
  • Distilled H2O
  • Nucleotide DNTP
  • 5x GoTaq buffer
  • Template DNA (B. Subtilis ATCC 6633, 1:1 and 1:2)
  • Forward and reverse primers

Protocol:

  • For the full protocol, please refer to Colony PCR in Protocol List.
Conditions in ThermoCycler:
  • Melting temperature, Tm used for Anneal step is 59°C.

Results:

  • Gel electrophoresis will be run tomorrow.

Conclusion:

Please refer to Lab book dated 27.7.2010.