Team:Newcastle/Non-target-environment kill switch

From 2010.igem.org

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(New page: =Kill switch= ==Holin-Lysozyme lysis== [https://2008.igem.org/Team:UC_Berkeley/LysisDevice UC Berkeley developed a Holin-Lysozyme lysis device in 2008]. ==mazEF== Found in strains of Es...)
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==mazEF==
==mazEF==
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Found in strains of Escherichia coli a Bacillus subtilis. Consists of two two adjacent genes, mazE and mazF, located downstream from the relA gene.
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Found in strains of ''Escherichia coli'' and ''Bacillus subtilis''.
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mazF encodes a stable toxin, MazF.  
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Consists of two two adjacent genes, mazE and mazF, located downstream from the relA gene.
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mazE encodes a labile antitoxin, MazE, degraded in vivo by the ATP-dependent ClpPA serine protease [13].
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mazF encodes a stable toxin, MazF.
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mazE encodes a labile antitoxin, MazE, degraded in vivo by the ATP-dependent ClpPA serine protease.
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MazE and MazF are coexpressed and mazEF is negatively autoregulated at the level of transcription by the combined action of both MazE and MazF proteins on the
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MazE and MazF are coexpressed and mazEF is negatively auto-regulated at the level of transcription by the combined action of both MazE and MazF proteins on the mazEF promoter P2.
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mazEF promoter P2.
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This system is activated by several stressful conditions that prevent the expression of mazEF, and thereby MazE synthesis, and thus trigger cell death. These conditions include: i) extreme amino acid starvation leading to the production of the starvation-signaling molecule ppGpp [13,42]; ii) inhibition of transcription and/or translation by antibiotics including rifampicin, chloramphenicol, and spectinomycin [43]; iii) Doc protein, a general inhibitor of translation which is the toxic product of the ‘‘addiction module’’ phd-doc of the plasmid prophage P1.
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This system is activated by several stressful conditions that prevent the expression of mazEF, and thereby MazE synthesis, and thus trigger cell death. These conditions include: i) extreme amino acid starvation leading to the production of the starvation-signaling molecule ppGpp ii) inhibition of transcription and/or translation by antibiotics including rifampicin, chloramphenicol, and spectinomycin iii) Doc protein, a general inhibitor of translation which is the toxic product of the ‘‘addiction module’’ phd-doc of the plasmid prophage P1.

Revision as of 17:14, 12 June 2010

Kill switch

Holin-Lysozyme lysis

UC Berkeley developed a Holin-Lysozyme lysis device in 2008.

mazEF

Found in strains of Escherichia coli and Bacillus subtilis. Consists of two two adjacent genes, mazE and mazF, located downstream from the relA gene. mazF encodes a stable toxin, MazF. mazE encodes a labile antitoxin, MazE, degraded in vivo by the ATP-dependent ClpPA serine protease.

MazE and MazF are coexpressed and mazEF is negatively auto-regulated at the level of transcription by the combined action of both MazE and MazF proteins on the mazEF promoter P2.

This system is activated by several stressful conditions that prevent the expression of mazEF, and thereby MazE synthesis, and thus trigger cell death. These conditions include: i) extreme amino acid starvation leading to the production of the starvation-signaling molecule ppGpp ii) inhibition of transcription and/or translation by antibiotics including rifampicin, chloramphenicol, and spectinomycin iii) Doc protein, a general inhibitor of translation which is the toxic product of the ‘‘addiction module’’ phd-doc of the plasmid prophage P1.

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