Team:HokkaidoU Japan/Notebook/September13

From 2010.igem.org

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(→GFP(1-12O)ゲル抽精製済の濃度測定)
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=Gel purification of GFP(1-12O) and concentration check=
=Gel purification of GFP(1-12O) and concentration check=
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[[Image:HokkaidoU Japan 20100913c.jpg‎|200px|right|thumb|]]
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[[Image:HokkaidoU Japan 20100913c.jpg‎|200px|right|thumb|Electrophoresis for concentration check]]
* [https://2010.igem.org/Image:HokkaidoU_Pictures_DNA_Marker.png TSUDA I] 2 uL, DNA 0.5 uL
* [https://2010.igem.org/Image:HokkaidoU_Pictures_DNA_Marker.png TSUDA I] 2 uL, DNA 0.5 uL

Revision as of 06:27, 28 September 2010

Notebook

araC promoter purification

Electrophoresed for gel extraction

Compared to marker band was a little lower than it should. Thinking that this was due to too big an amount, gel extracted anyway.

  • Used TSUDA marker
  • Part length is 1259 bp

Electrophoresed after gel extraction

Electrophoresis after purification
  • Electrophoresed TSUDA ITSUDA I 2 uL and 0.5 uL of purified solutuion

→Estimated concentration to be 54 ng/uL

  • This time band location is good
  • Accidentally excised a part of other band resulting small contamination

Gel purification of GFP(1-12O) and concentration check

Electrophoresis for concentration check

→Estimated concentration to be 120 ng/uL

  • Part length is 878 + 220 bp = 947 bp
  • That slightly above mark so OK．
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