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Team:HokkaidoU Japan/Notebook/August20
From 2010.igem.org
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| - | = | + | =Amplified vector by PCR= |
| + | |||
[[Image:HokkaidoU Japan 20100820a.JPG|200px|right|thumb|]] | [[Image:HokkaidoU Japan 20100820a.JPG|200px|right|thumb|]] | ||
| - | pSB1C3, pSB1T3, | + | |
| + | Amplified pSB1C3, pSB1T3, pSB1A3 accordingly to the table bellow. | ||
| + | |||
{| class="protocol" | {| class="protocol" | ||
|'''Reagent''' | |'''Reagent''' | ||
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|} | |} | ||
| - | * | + | * pSB1T3 failed to amplify |
Revision as of 17:38, 21 September 2010
since 13 Jul 2010
since 1 Aug 2010
Amplified vector by PCR
Amplified pSB1C3, pSB1T3, pSB1A3 accordingly to the table bellow.
| Reagent | Amount |
| DW | 34 uL |
| 10x buffer | 5 uL |
| 2mM dNTP | 5 uL |
| 25mM MgSO4 | 3 uL |
| Suffix-F | 0.5 uL |
| Prefix-R | 0.5 uL |
| KOD | 1 uL |
| DNA Template | 1 uL |
| Total | 50 uL |
- pSB1T3 failed to amplify
