Team:EPF Lausanne/Notebook/week9

From 2010.igem.org

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== Cloning ==
== Cloning ==
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*Western Blot from yesterday repeated. Colony PCR done to check if we are looking at the cells containing the correct plasmid.  
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*Western Blot from yesterday repeated. Same result as yesterday.  
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== Drosophila ==
== Drosophila ==

Revision as of 11:06, 13 September 2010

Contents

06-09-2010

Cloning

  • Western Blot started of C3-S-Immunotoxin to check if the Immunotoxin is expressed.

Drosophila

07-09-2010

Cloning

  • Western Blot from yesterday finished. Observed one band which did NOT correspond to the right size. Furthermore the negative control failed! Reason not known. Maybe it is another protein with a His-tag which is present on the C3 plasmid.

Drosophila

Other 

*Gwen made us a chocolate cake!! Yeah!

08-09-2010

Cloning

  • Western Blot from yesterday repeated. Same result as yesterday.

Drosophila

Other 

*Solange has a hangover! ;)
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