Team:Newcastle/16 August 2010
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# We then find out the concentration of resuspended ''yneA'' from [[TeamNewcastleNanoDrop_Spectrophotometer|nanodrop]]. | # We then find out the concentration of resuspended ''yneA'' from [[TeamNewcastleNanoDrop_Spectrophotometer|nanodrop]]. | ||
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- | + | =Transformation of ''E. coli''= | |
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+ | ==Aim== | ||
+ | |||
+ | Transforming ''yneA'' into ''E. coli'' so that we have plenty of stocks to future tests. | ||
+ | |||
+ | ==Materials and Protocol== | ||
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+ | Please refer to [[Team:Newcastle/Transformation_of_E._coli|transformation of ''E. coli'']] | ||
+ | |||
+ | ==Results and Conclusion== | ||
+ | |||
+ | Please refer to [[Team:Newcastle/17_August_2010|17.08.10]]. | ||
'''Go back to our main [[Team:Newcastle/notebook| Lab book]] page''' | '''Go back to our main [[Team:Newcastle/notebook| Lab book]] page''' |
Revision as of 08:46, 17 August 2010
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Contents |
Rehydrating yneA
Aim
To get yneA hydrated so that we can run restriction digest (see below).
Methods
- The dehydrated DNA is resuspended in 0.5ml of water.
- We then find out the concentration of resuspended yneA from nanodrop.
Transformation of E. coli
Aim
Transforming yneA into E. coli so that we have plenty of stocks to future tests.
Materials and Protocol
Please refer to transformation of E. coli
Results and Conclusion
Please refer to 17.08.10.
Go back to our main Lab book page