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Team:Osaka/notebooktest
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Revision as of 14:13, 15 August 2010
July 29 (Thu)
Attendance: Torigata, Takino, Teoh, Yasumoto, Kakuda, Saka, Tamura
# Safety lecture for junior members.
# Preparation of LB agar plates (26 Amp, 25 Kan, 25 Cam).
July 31 (Thu)
Attendance: Miyatake, Hirayama, Torigata, Teoh, Tadashi, Yasumoto, Kakuda, Saka
# Meeting
#* Summer project schedule
#* List of genes to clone
August 2 (Mon)
Attendance: Torigata, Takino, Teoh, Tadashi, Yasumoto, Saka
# Culture medium preparation
#* LB agar plates (49 antibiotic-less plates)
#* LB liquid medium (500 ml)
# Competent cells preparation - Nojima Method
#* SOB medium (MgCl2 not yet added) -> stored at 4˚C
#* TB buffer -> stored at 4˚C
#* Single-colony streaking of E. coli (strain DH5α) on 2 LB agar plates -> 37˚C incubation o/n
Tomorrow we shall prepare the remainder of the reagents and inoculate SOB with o/n-incubated E. coli.
