Team:Newcastle/28 July 2010
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===Materials and Protocol=== | ===Materials and Protocol=== | ||
+ | Please refer to: [[Team:Newcastle/PCR| PCR]] for GoTaq PCR. | ||
===Result=== | ===Result=== |
Revision as of 14:48, 28 July 2010
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Contents |
Aims
The aim of this experiment is to prove that Bacillus subtilis 168 and 3610 DNA extraction worked by amplifying anaR gene using PCR.
Materials and Protocol
Please refer to: PCR for GoTaq PCR.
Result
Discussion
Arginine consists of a positive charge on it and thus is acidic in nature. As the cells take up the arginine from the media, the pH of the media increases. The tube labeled Negative control contains no cells and the pH of the media has remained constant for 5 hours which shows that the level of arginine in the media has remained constant, while the tube labeled Test which contains Bacillus subtilis 168 cells shows an increase in pH which shows that the level of arginine has decreased in the media.
Conclusion
It can be concluded that the Bacillus subtilis 168 cells are able to take up arginine from the media into the cells.