Team:Stanford/Notebook/Lab Work/Week 1

From 2010.igem.org

(Difference between revisions)
(→June 28)
(→June 28)
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=====June 28=====
=====June 28=====
 +
General Meeting
 +
*Wiki stuff
 +
**Everyone should sign up on 2010.igem.org website
 +
***Take advantage of the new calendar system
 +
***Need to transfer current website over
 +
**Current wiki needs a new vision
 +
***Francisco + Alex assembling wiki committee
 +
*Transform the first wave of cells
 +
**Pick colonies
 +
**Colony PCR
 +
**Sequence parts to validate
 +
***Use the Smolke sequencing network
 +
*Characterize promoters
 +
**Digest, ligate
 +
**Need to acquire enzymes
 +
**Need an PO account.
 +
***Email Jeanne
 +
*Get competent cells
 +
*Getting DNA
 +
**Assembly PCR
 +
**PCR from host cells
 +
**Synthesize de novo
 +
*RNA team
 +
**Use RNAstructure
 +
***Maybe use ViennaRNA or dinamelt
 +
**Talk to Arkin and Berkeley Postdocs
 +
**Finalize sRNA library
 +
**9kb plasmid
 +
*
 +
*Meeting scheudle
 +
**Monday morning is good for logistics
 +
**Group meetings for presentations
 +
***One person presents their work in the context of everything else
 +
**Journal club
 +
**Karina will set up a doodle

Revision as of 21:26, 19 July 2010

June 28

General Meeting

  • Wiki stuff
    • Everyone should sign up on 2010.igem.org website
      • Take advantage of the new calendar system
      • Need to transfer current website over
    • Current wiki needs a new vision
      • Francisco + Alex assembling wiki committee
  • Transform the first wave of cells
    • Pick colonies
    • Colony PCR
    • Sequence parts to validate
      • Use the Smolke sequencing network
  • Characterize promoters
    • Digest, ligate
    • Need to acquire enzymes
    • Need an PO account.
      • Email Jeanne
  • Get competent cells
  • Getting DNA
    • Assembly PCR
    • PCR from host cells
    • Synthesize de novo
  • RNA team
    • Use RNAstructure
      • Maybe use ViennaRNA or dinamelt
    • Talk to Arkin and Berkeley Postdocs
    • Finalize sRNA library
    • 9kb plasmid
  • Meeting scheudle
    • Monday morning is good for logistics
    • Group meetings for presentations
      • One person presents their work in the context of everything else
    • Journal club
    • Karina will set up a doodle
Retrieved from "http://2010.igem.org/Team:Stanford/Notebook/Lab_Work/Week_1"