Team:Stanford/NotebookPage/9 July 2010

From 2010.igem.org

(Difference between revisions)
(Karina's Notebook)
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== Karina's Notebook ==
== Karina's Notebook ==
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Today's Goal: Digest GFP, RFP, and Terminators. Will ligate (GFP + T) and  (RFP + T) on monday. <br/>
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Today's Goal: Digest GFP, RFP, and Terminators. Will ligate (GFP + T) and  (RFP + T) on monday. <br/><br/>
-
Part #'s:
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'''Part #'s:''' <br/>
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GFP
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GFP: E0040 (cut at S and P) <br/>
 +
RFP: E1010 (cut at S and P) <br/>
 +
Term: B1006 (cut at X and P) <br/><br/><br/>
 +
 
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'''Recipe'''
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12.0 uL DNA<br/>
 +
1.0 uL each enzyme<br/>
 +
5.0 uL NEB buffer 2<br/>
 +
5 uL BSA (10x) <br/>
 +
Sterile H20 to fill up to 50 uL

Revision as of 18:57, 19 July 2010

Meeting Minutes

[http://docs.google.com/present/edit?id=0Ae31et9w_tAhZGZyc3R2ejJfOGNnY2drMmZz&hl=en&authkey=CPHGzfIB Weekly Leader Presentation: Karina]

Agenda

Karina's Notebook

Today's Goal: Digest GFP, RFP, and Terminators. Will ligate (GFP + T) and (RFP + T) on monday.

Part #'s:
GFP: E0040 (cut at S and P)
RFP: E1010 (cut at S and P)
Term: B1006 (cut at X and P)


Recipe 12.0 uL DNA
1.0 uL each enzyme
5.0 uL NEB buffer 2
5 uL BSA (10x)
Sterile H20 to fill up to 50 uL