Team:EPF Lausanne/SecondWeek
From 2010.igem.org
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*Autoclaving of media and flasks | *Autoclaving of media and flasks | ||
- | <h3>Tuesday | + | <h3>Tuesday 20.07.2010 </h3> |
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+ | <h3>Wednesday 21.07.2010 </h3> | ||
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+ | <h3>Thursday 22.07.2010 </h3> | ||
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+ | <h3>Friday 23.07.2010 </h3> |
Revision as of 14:43, 19 July 2010
Contents |
Monday 19.07.2010
- Results of the transformation checked: successful!!
- Amplification of colonies (goal to do a miniprep of the plasmids --ori-- and --ori-resistance--)
- Results of plating of asaia culture (where we hope to recover the WT) checked: Asaia cultures grew on Kan plates also! Checked colonies for fluorescance under the microscope: no fluorescance --> Very unexpected! Don't know reason yet.
- Re-grow the colonies. Multiple cycles: hope that we get WT.
- Preparation of Asaia cultures for Lemaitre experiments/WT/Competence...
- Preparation of SOC medium and GLY medium
- Autoclaving of media and flasks