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Team:EPF Lausanne/Protocols
From 2010.igem.org
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== Lab Protocols == | == Lab Protocols == | ||
On this page we collect summaries of the procedures we have learned in the lab. These protocols are kept in a very simple non-scientific language since they should also be understood quickly by the non-biology students. They are specific to the lab of professor Maerkl where the iGEM team is working this summer. The protocols should enable the new team members in the lab to learn procedures quickly so that we don't have to bother Henrike and the other TA's too much. | On this page we collect summaries of the procedures we have learned in the lab. These protocols are kept in a very simple non-scientific language since they should also be understood quickly by the non-biology students. They are specific to the lab of professor Maerkl where the iGEM team is working this summer. The protocols should enable the new team members in the lab to learn procedures quickly so that we don't have to bother Henrike and the other TA's too much. | ||
| + | * adapt ph (Nadine) | ||
*[https://static.igem.org/mediawiki/2010/6/6f/Prot_agarose_gel.pdf Agarose Gel protocol] | *[https://static.igem.org/mediawiki/2010/6/6f/Prot_agarose_gel.pdf Agarose Gel protocol] | ||
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*[https://static.igem.org/mediawiki/2010/6/63/Prot_agar_plates.pdf Agar plates production protocol] | *[https://static.igem.org/mediawiki/2010/6/63/Prot_agar_plates.pdf Agar plates production protocol] | ||
| - | * | + | *[https://static.igem.org/mediawiki/2010/f/f9/Prot_autoclave.pdf Autoclave protocol] |
*[https://static.igem.org/mediawiki/2010/8/82/Prot_electrophoresis.pdf Electrophoresis] | *[https://static.igem.org/mediawiki/2010/8/82/Prot_electrophoresis.pdf Electrophoresis] | ||
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* Asaia Maxiprep (effie) | * Asaia Maxiprep (effie) | ||
* Asaia Miniprep (effie) | * Asaia Miniprep (effie) | ||
| - | * [https://static.igem.org/mediawiki/2010/6/6a/Prot_ligation.pdf Ligation protocol] | + | *[https://static.igem.org/mediawiki/2010/6/6a/Prot_ligation.pdf Ligation protocol] |
| - | * [https://static.igem.org/mediawiki/2010/ | + | *[https://static.igem.org/mediawiki/2010/f/f1/Prot_nanodrop.pdf Measuring DNA concentration using NanoDrop] |
| + | *[https://static.igem.org/mediawiki/2010/e/ec/Prot_OD.pdf OD protocol] | ||
| + | *[https://static.igem.org/mediawiki/2010/6/6c/Prot_PCR.pdf PCR protocol ] | ||
* purification --> purification Kit | * purification --> purification Kit | ||
| + | *[https://static.igem.org/mediawiki/2010/1/1e/Prot_transformation.pdf Transformation of competent cells protocol ] | ||
| + | * UV imaging (Wiktor) | ||
| + | * Using the plate reader (Solange) | ||
Media Recipes | Media Recipes | ||
Revision as of 12:50, 19 July 2010
Lab Protocols
On this page we collect summaries of the procedures we have learned in the lab. These protocols are kept in a very simple non-scientific language since they should also be understood quickly by the non-biology students. They are specific to the lab of professor Maerkl where the iGEM team is working this summer. The protocols should enable the new team members in the lab to learn procedures quickly so that we don't have to bother Henrike and the other TA's too much.
- adapt ph (Nadine)
- Agarose Gel protocol
- Agar plates production protocol
- Autoclave protocol
- Electrophoresis
- Asaia Maxiprep (effie)
- Asaia Miniprep (effie)
- Ligation protocol
- Measuring DNA concentration using NanoDrop
- OD protocol
- PCR protocol
- purification --> purification Kit
- Transformation of competent cells protocol
- UV imaging (Wiktor)
- Using the plate reader (Solange)
Media Recipes
Syntax: convention if you want to upload a new protocol
When uploading a file it please rename it in the following way: prot_yourProcedureName.yourExtension.
