Team:Panama
From 2010.igem.org
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- | <p>Our aim is to construct a BioBrick that leads to the production of rhlAB enzyme complex in <span style="font-style: italic;">E.coli</span>. To accomplish this we must modify the natural gene sequence of rhlAB complex because it includes illegal restriction sites and requires modifications to be fit into a BioBrick.We will perform silent mutations in the gene sequence with a form of mutagenesis. To measure our success we will do bio-assays with our new<span style="font-style: italic;">E. coli rhlAB</span> strain to degrade rhamnose sugars and fatty-acids substrates and turn it in rhamnolipids. We hope that our BioBricks will become a useful tool for the bioengineered industry and widely used as a simple and effective means of rhamnolipid production.</p> | + | <p>Our aim is to construct a BioBrick that leads to the production of rhlAB enzyme complex in <span style="font-style: italic;">E.coli</span>. To accomplish this we must modify the natural gene sequence of rhlAB complex because it includes illegal restriction sites and requires modifications to be fit into a BioBrick.We will perform silent mutations in the gene sequence with a form of mutagenesis. To measure our success we will do bio-assays with our new <span style="font-style: italic;">E. coli rhlAB</span> strain to degrade rhamnose sugars and fatty-acids substrates and turn it in rhamnolipids. We hope that our BioBricks will become a useful tool for the bioengineered industry and widely used as a simple and effective means of rhamnolipid production.</p> |
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Revision as of 05:55, 16 July 2010