Team:Newcastle/Meetings/12 July 2010
From 2010.igem.org
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In charge of wiki - Deena and Rachel (everyone everyday they have to send the links to both of them to check) | In charge of wiki - Deena and Rachel (everyone everyday they have to send the links to both of them to check) | ||
- | # | + | #'''Presentation''' |
- | Introduction of our iGEM project - using BS to repair concrete | + | Cover Page |
+ | Picture of standing us up | ||
+ | Introduction of our iGEM project - using BS to repair concrete (2 mins) | ||
+ | |||
+ | |||
+ | '''General Outlook''' (5 mins): | ||
Starting with bioreactor | Starting with bioreactor | ||
Transformation into spores | Transformation into spores | ||
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Filamentous cells | Filamentous cells | ||
CaCO3 | CaCO3 | ||
+ | Glue | ||
+ | Kill Switch | ||
+ | |||
+ | '''BioBrick Discussion''' (8 mins altogether; 2 mins each): | ||
+ | Swarming | ||
+ | Subtillin | ||
+ | Filamentous cells - YneA | ||
+ | CaCo3 - Urease | ||
+ | |||
+ | (5 mins remaining) | ||
+ | |||
+ | '''Whats next'''... |
Revision as of 15:26, 12 July 2010
Chair: Da, Minutes: Younus, Comp
- . Cloning Strategies
- SR1 - Finished
- RocF not started - need to use Gibson method;
- YneA
- 2 for Swarming (sfp and swrA)
- LacI
- 2 for Subtilin (Immunity and Production)
Only finished one, SR1. 7 more to go!
Next: swarming and subtilin nearly finished, YneA nearly coming back and LacI brick not sure how to test. Why is testing LacI hard?
- YneA - our most important BB - Phil, Rach, Deena, Younus
- Swarming (2) - Harsh, Alan, Steve, Da
- LacI - Phil, Younus, Rachel
- Subtilin (2) - Harsh, Alan, Steve, Deena
- RocF - Harsh, Alan, Steve, Da
5 parts to a Cloning Strategy
- Purpose and justification
- Modelling
- Construction
- Cloning and Integration
- Testing and Characterising
- . Wiki
Labbook
- . Aim
- . Materials and Methods
- . Procedure/Protocol
- . Results
- . Conclusion
Everyone needs to carry out this protocol above Photographs add and label them In charge of wiki - Deena and Rachel (everyone everyday they have to send the links to both of them to check)
- Presentation
Cover Page Picture of standing us up Introduction of our iGEM project - using BS to repair concrete (2 mins)
General Outlook (5 mins):
Starting with bioreactor
Transformation into spores
Transportation
Spraying on building
Germination
sensing crack + non-environmental kill switch
swarming
end of crack signalling = QS
Filamentous cells
CaCO3
Glue
Kill Switch
BioBrick Discussion (8 mins altogether; 2 mins each): Swarming Subtillin Filamentous cells - YneA CaCo3 - Urease
(5 mins remaining)
Whats next...