Team:Queens-Canada/17 June 2010
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(Difference between revisions)
(New page: <h2>June 17, 2010</h2> <h3>Gel Electrophoresis</h3> Kevin and Nelson '''pSip-1'''::'''eGFP''', '''pHsp-3'''::'''eGFP''', '''pMec-7''':...) |
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<h3>[[Team:Queens-Canada/protocols#Gel Electrophoresis|Gel Electrophoresis]]</h3> | <h3>[[Team:Queens-Canada/protocols#Gel Electrophoresis|Gel Electrophoresis]]</h3> | ||
Yuli | Yuli | ||
- | + | PCR Purification products from June 16, 2010 | |
Used 0.4 g agarose in in 60 mL TBE. Gel run for 40 mins at 100 V. | Used 0.4 g agarose in in 60 mL TBE. Gel run for 40 mins at 100 V. |
Revision as of 05:23, 27 October 2010
June 17, 2010
Gel Electrophoresis
Kevin and Nelson
pSip-1::eGFP, pHsp-3::eGFP, pMec-7::eGFP, pUnc-47::eGFP, pRab-7::eGFP
Used 1.1 g agarose in 160 mL TBE. Gel run for 40 mins at 100 V.
2010 Digestion Nelson
BBa_J45200, BBa_J45120
Incubated at 37 °C for 20 mins. Products stored at -20 °C.
Gel Electrophoresis
Yuli PCR Purification products from June 16, 2010
Used 0.4 g agarose in in 60 mL TBE. Gel run for 40 mins at 100 V.