Team:Newcastle/3 September 2010
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==Conclusion== | ==Conclusion== | ||
- | The results show that the digest works, there is a band at 541bp corresponding to ''yneA'' and a band at 8.4kbp corresponding to | + | The results show that the digest works, there is a band at approximately 541bp corresponding to ''yneA'' and a band at approximately 8.4kbp corresponding to pGFPrrnb in lanes 2,3,4,6,7,8,9, 11 and 12. |
{{Team:Newcastle/footer}} | {{Team:Newcastle/footer}} |
Revision as of 23:52, 25 October 2010
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Contents |
yneA
Aims
The aim of the experiment is to test for the correct integration of yneA in pGFPrrnb plasmid.
Materials and protocols
- Please refer to: Plasmid extraction.
- Please refer to:Restriction digest. We used Nhe1 and Spe1 to remove the yneA from pGFP-rrnB.
- Please refer to: Gel electrophoresis for running all the digested plasmid fragments.
Results
Conclusion
The results show that the digest works, there is a band at approximately 541bp corresponding to yneA and a band at approximately 8.4kbp corresponding to pGFPrrnb in lanes 2,3,4,6,7,8,9, 11 and 12.