Team:EPF Lausanne/Project

From 2010.igem.org

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So... ASAIA is the pink power against malaria.....
So... ASAIA is the pink power against malaria.....
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= Movie =
= Movie =

Revision as of 21:40, 24 October 2010



Contents

Overview

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Our projects aims to help stopping the propagation of malaria.

Mousquito.png To do so, we chose to act on the mosquito. Indeed, if we prevent the mosquito from being infected by malaria, it won't be able to transmit the parasite to healthy humans.

Mousquito and asaia 3.png Gut.png Our idea is to engineer asaia, a bacteria that is naturally present in the mosquito's intestinal tract. All team.png This bacteria would express an immunotoxin to kill the plasmodium and thus prevent the mosquito infection. Hunting.png

So... ASAIA is the pink power against malaria..... File:Image:Zorro.png

Movie

For a playful overview of our project, you can watch our great iGEM EPFL movie

Plan

After choosing our bacteria of interest, here Asaia, we decided on two main set of experiments.

Cloning

The first, of course, was the cloning part. We performed all the cloning in E.coli, and did experiments on Asaia transformation, growth and resistance to antibiotics in parallel.

Here are the parts we successfully cloned.

As E.Coli origin does not work in Asaia, we added an origin that was compatible with this bacteria. Gwen: where did we get this origin from? On the opposite, Asaia's origin does work in E.Coli, which is very useful for our sets of experiments.

dessin des "parts"

Asaia Characterization

We measured the doubling time of Asaia.

Graph growth curve2.jpg

We tested our bacteria with 9 differents antibiotics and measured the resistance with a paper filter test and a liquid culture test.


Graph Resistance2.jpg




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