Team:EPF Lausanne/Project droso

From 2010.igem.org

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===Drosophila===
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In parallel, we did experiments to assess if Asaia was persistant in ''[https://2010.igem.org/Team:EPF_Lausanne/Project_droso Drosophila] melanogaster ''. In fact, the fly is a much easier organism to grow and work on, in opposition to mosquitoes which need to feed on animals to reproduce.
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Our experiment was set in 3 steps:
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*infection of the flies with GFP-Asaia*, and 3 other bacteria as controls
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*retrieval of the flies after 3h (control of infection), 24h, 48h
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*counting of the number of colonies for all time points and the 4 differents strains
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The goal was to observe the persistance of Asaia in the Drosophila gut to see if it can be used as a model organism to study Asaia.
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The results were negative for a the experience and we repeteated it 4 times.
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''graphe des drosophiles''
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Revision as of 17:11, 24 October 2010



Drosophila

In parallel, we did experiments to assess if Asaia was persistant in Drosophila melanogaster . In fact, the fly is a much easier organism to grow and work on, in opposition to mosquitoes which need to feed on animals to reproduce. Our experiment was set in 3 steps:

  • infection of the flies with GFP-Asaia*, and 3 other bacteria as controls
  • retrieval of the flies after 3h (control of infection), 24h, 48h
  • counting of the number of colonies for all time points and the 4 differents strains

The goal was to observe the persistance of Asaia in the Drosophila gut to see if it can be used as a model organism to study Asaia.

The results were negative for a the experience and we repeteated it 4 times.

graphe des drosophiles