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Minnesota-experimental/20 July 2010
From 2010.igem.org
(Difference between revisions)
(New page: '''Annie''' Made YDP media and inoculated PIchia Pastoris. '''Tony''' PacBB GGFP digest with BglII/NotI. Cut out top band and saved for purification tomorrow. Transformation of pacBB Eut...) |
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'''Tony''' | '''Tony''' | ||
PacBB GGFP digest with BglII/NotI. Cut out top band and saved for purification tomorrow. Transformation of pacBB Eutc DsRed ligation into JM109. | PacBB GGFP digest with BglII/NotI. Cut out top band and saved for purification tomorrow. Transformation of pacBB Eutc DsRed ligation into JM109. | ||
| + | '''Matt''' | ||
| + | Minipreps of pacBB EutC DsRed. | ||
| + | '''Tony''' | ||
| + | Preparation of pacBB EutC DsRed for sequencing. Overnight cultures of psB3K3 Eut LKMNS. | ||
Latest revision as of 00:34, 24 October 2010
Annie Made YDP media and inoculated PIchia Pastoris. Tony PacBB GGFP digest with BglII/NotI. Cut out top band and saved for purification tomorrow. Transformation of pacBB Eutc DsRed ligation into JM109. Matt Minipreps of pacBB EutC DsRed. Tony Preparation of pacBB EutC DsRed for sequencing. Overnight cultures of psB3K3 Eut LKMNS.
