Team:SDU-Denmark/project-r
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'''UV-Vis spectrophotometer determination of beta-carotene production:'''<br> | '''UV-Vis spectrophotometer determination of beta-carotene production:'''<br> | ||
In this experiment cells was prepare and harvested according to protocol [https://2010.igem.org/Team:SDU-Denmark/protocols#EX1.1]. This experiment was preformed with four different strain of E. Coli – wildtype Top10, wildtype MG1655, Top10 containing PSB1A2 with K274210 constitutively active and MG1655 containing PSB1A2 with K274210 constitutively active. The resulting graphs is presented beneath the text<br> | In this experiment cells was prepare and harvested according to protocol [https://2010.igem.org/Team:SDU-Denmark/protocols#EX1.1]. This experiment was preformed with four different strain of E. Coli – wildtype Top10, wildtype MG1655, Top10 containing PSB1A2 with K274210 constitutively active and MG1655 containing PSB1A2 with K274210 constitutively active. The resulting graphs is presented beneath the text<br> | ||
- | [[Image:Team-SDU- | + | [[Image:Team-SDU-denmarkBetacarotene samples with controles (exponential_phase).png |250px|thumb|Wildtype bacteria exposed to blue light]] |
- | [[Image:Team-SDU- | + | [[Image:Team-SDU-denmarkBetacarotene samples with controles (stationary phase).png |250px|thumb|Wildtype bacteria exposed to blue light]] |
In order assess the results a standard dilution of beta-carotene: 1mM, 100 µM, 50 µM, 25 µM, 10 µM, 5 µM, 1 µM, 100 nM and 10 nM. The standart dilutions was also measured on the spectrophotometer. The resulting graphs is presented beneath the text<br> | In order assess the results a standard dilution of beta-carotene: 1mM, 100 µM, 50 µM, 25 µM, 10 µM, 5 µM, 1 µM, 100 nM and 10 nM. The standart dilutions was also measured on the spectrophotometer. The resulting graphs is presented beneath the text<br> | ||
- | [[Image:Team-SDU- | + | [[Image:Team-SDU-denmarkStandart betacaroten curves.png |250px|thumb|Wildtype bacteria exposed to blue light]] |
When combining the graphs of the samples and the standard dilutions it is possible to tell that the spectres are uniform. This indicates that it is Beta-carotene which is produced because of the similarity in the spectres. The graphs also indicate the amount of Beta-carotene produced by the MG1655 and the Top10 E. Coli cells. The resulting graphs is presented beneath the text<br> | When combining the graphs of the samples and the standard dilutions it is possible to tell that the spectres are uniform. This indicates that it is Beta-carotene which is produced because of the similarity in the spectres. The graphs also indicate the amount of Beta-carotene produced by the MG1655 and the Top10 E. Coli cells. The resulting graphs is presented beneath the text<br> |
Revision as of 11:22, 18 October 2010