Team:LMU-Munich/Notebook/Protocols/3 Transformation
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(New page: {{:Team:LMU-Munich/Templates/Page Header}} Transformation (Source: http://openwetware.org/wiki/Transforming_chemically_competent_cells) 1. Thaw TSS cells on ice. 2. Add DNA, pipette ...) |
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- | hugh kingston 09:46, 4 February 2008 (CST):I tried a few variations on this protocol, and found | + | |
- | + | hugh kingston 09:46, 4 February 2008 (CST):I tried a few variations on this protocol, and found | |
- | + | using SOC instead of LB + 20mM glucose increased efficiency 3 fold | |
+ | heat shock of 42°C 45s increased efficiency 15-20 fold compared to no heat shock | ||
{{:Team:LMU-Munich/Templates/Page Footer}} | {{:Team:LMU-Munich/Templates/Page Footer}} |
Latest revision as of 17:31, 11 August 2010