Team:TU Delft/22 July 2010 content

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(Alkane degradation)
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[[Image:TUDelft_scPCR_22-07.png|400px|thumb|left|1% Agarose gel of SC PCR samples]]
[[Image:TUDelft_scPCR_22-07.png|400px|thumb|left|1% Agarose gel of SC PCR samples]]
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Revision as of 07:38, 23 July 2010

Alkane degradation

There were some colonies on Tuesday's plates! We had left the plates @ 37°C yesterday after having seen that there were no colonies. When checking this morning on all plates (except the negative control) there were a few colonies! (2-50 colonies). Chances are it's not what we're looking for, but maybe they are good transformants... to check we will do a colony PCR.

1% Agarose gel of SC PCR samples
# Description Expected length (bp)
1 [SmartLadder] (5 μL) n/a
2 007T 1616
3 007T 1616
4 007T 1616
5 008T 551
6 008T 551
7 009T 560
8 009T 560
9 009T 560
10 010T 1657
11 010T 1657
12 010T 1657
13 017T 1130
14 017T 1130
15 017T 1130
16 018T 1874
17 Red colony n/a