Team:Stanford/Research

From 2010.igem.org

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*Allow future users to change output proteins and input promoters without disrupting the sensor's internal machinery
*Allow future users to change output proteins and input promoters without disrupting the sensor's internal machinery
*Have both sensors be orthogonal to the host system to avoid cross-talk  
*Have both sensors be orthogonal to the host system to avoid cross-talk  
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==Important Parameters==
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===Digital Sensor===
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#Range of possible ratios
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#Background expression level
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##Of both proteins at ratio
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##Of either protein when it shouldn't be being expressed
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#Response curve
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#Response time
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===Analog Sensor===
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#Range of possible ratios
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#Background expression level (no kinase-inducing chemical added)
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#Phosphotase specificity
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#Response curve
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#Response time

Revision as of 18:10, 22 July 2010

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Contents

Project Goals

Digital Sensor

  • Accurately report whether the concentrations of input chemicals lie at, above, or below a given ratio
  • Allow as little expression as possible at the detection ratio, and as much as possible at all other ratios


Analog Sensor

  • Accurately report over a large range of input ratios
  • Achieve a linear correlation between output protein concentration and detection ratio
  • Have a low basal level of expression


Both Sensors

  • Allow future users to change output proteins and input promoters without disrupting the sensor's internal machinery
  • Have both sensors be orthogonal to the host system to avoid cross-talk


Important Parameters

Digital Sensor

  1. Range of possible ratios
  2. Background expression level
    1. Of both proteins at ratio
    2. Of either protein when it shouldn't be being expressed
  3. Response curve
  4. Response time


Analog Sensor

  1. Range of possible ratios
  2. Background expression level (no kinase-inducing chemical added)
  3. Phosphotase specificity
  4. Response curve
  5. Response time


Research

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