Team:TU Delft/1 July 2010 content

From 2010.igem.org

(Difference between revisions)
Line 7: Line 7:
|'''medium'''
|'''medium'''
|'''day 1 = 30 June'''
|'''day 1 = 30 June'''
-
|'''day 2'= 1 July''
+
|'''day 2= 1 July'''
|-
|-
|no cells
|no cells
Line 60: Line 60:
 +
<h4>Competent cells</h4>
Top10 cells were cultivated for making competent cells.
Top10 cells were cultivated for making competent cells.

Revision as of 18:18, 17 July 2010

Lab Work

Pseudomonas Putida GPO1

The second day of the grow process of Pseudomonas Putida GPO1

Sample medium day 1 = 30 June day 2= 1 July
no cells E2 medium without carbon 0.00 0.00
no cells LB medium 0.00 0.00
no cells E2 + 1% LB 0.00 0.01
Pseudomonas Putida GPO1 * E2 + 1% LB 0.01 0.05
no cells E2 + 10% LB 0.00 0.00
Pseudomonas Putida GPO1 * E2 + 10% LB 0.34 0.19
no cells E2 + 1% octane 0.00 0.00
Pseudomonas Putida GPO1 * E2 + 1% octane 0.00 0.00
Pseudomonas Putida GPO1 * E2 + 10% octane 0.29 0.18

* average of 3 colonies

Pseudomonas Putida GPO1 can grow on 10% octane as carbon source.


Competent cells

Top10 cells were cultivated for making competent cells.