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Team:Mexico-UNAM-CINVESTAV/Notebook/Week Seven
From 2010.igem.org
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| + | '''We made the ligation with the one hour amresco kit and transformed in 4 plates''' | ||
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| + | '''Aditional to this tried to ligate with Fermentas T4 this reaction is overnight at 4°''' | ||
==''Thursday''== | ==''Thursday''== | ||
==''Friday''== | ==''Friday''== | ||
Revision as of 22:59, 27 October 2010
Week #7
18th October - 22th October 2010
Monday
We prepared inoculum in 6 falcon tubes of E0240 and I20260 each, after
nano espectophotometer readings we are going to need huge amounts of plasmid
Tuesday
With yesterday's inoculum we prepared six vials of culture to do miniprep and extracted from the band
we ran a agarosa's gel with 16μl per well, then precipitated with salts and alcohol the DNA and kept
overnight at -20°C'
Wednesday
We made the ligation with the one hour amresco kit and transformed in 4 plates
Aditional to this tried to ligate with Fermentas T4 this reaction is overnight at 4°
