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From 2010.igem.org

Revision as of 10:22, 16 October 2010 (view source) Mio (Talk | contribs) (→2:Plasmid extraction from E-coli) ← Older edit		Revision as of 10:23, 16 October 2010 (view source) Mio (Talk | contribs) (→5：DNA Ligation) Newer edit →
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	===5：DNA Ligation ===		===5：DNA Ligation ===
	＜Member＞<br />		＜Member＞<br />
-	mio,mizuki	+	nito
	＜Sample＞<br />		＜Sample＞<br />
	*1-2		*1-2
-	*4-5	+	*10-2
-	*6A-7	+
-	*7-8-10	+
-	(after digestion)	+
	＜Protocol＞<br />		＜Protocol＞<br />

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Revision as of 10:23, 16 October 2010

Contents 1 2010/10/12 1.1 1：DNA extraction 1.2 2：DNA Digestion 1.3 3:PCR 1.4 4:Plasmid extraction from E-coli 1.5 5：DNA Ligation 1.6 3：DNA Digestion

2010/10/12

1：DNA extraction

＜Member＞
Mariko

＜Sample＞

• 1-2
• 10-2
• 2

＜Protocol＞
See Protocol 4

2：DNA Digestion

＜Member＞
Mariko

＜Sample, Materials＞
・PCR product

• 1-2

・Digest enzyme

• Avr2

＜Protocol＞
See Prptpcpl 9

3:PCR

<Member>
nito

<Sample>

• 9
• 1-2-3
• 4-5

<Protocol>
See Protocol 2

4:Plasmid extraction from E-coli

<Member>
taka

<Sample>

• 1-2
• 1-3
• 1-5
• 1-6

<Protocol>
See Protocol 6

5：DNA Ligation

＜Member＞
nito

＜Sample＞

• 1-2
• 10-2

＜Protocol＞
See Protocol 10

3：DNA Digestion

＜Member＞

＜Sample, Materials＞
・PCR productions

• 1L(8/25) 8μl
• 2L(8/26) 8μl
• 4H(8/26) 16μl
• 5H(8/26) 16μl

・Digest enzyme

• AvrⅡ
• NheⅠ
• SpeⅠ

＜Protocol＞
See Protocol 9