Team:Michigan/Oil Sands
From 2010.igem.org
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'''Transformation of pBAD''' | '''Transformation of pBAD''' | ||
- | Marcus and Alex tried the transformation of pBAD again according to the electroporation | + | Marcus and Alex tried the transformation of pBAD again according to the electroporation protocol with Ann's Notes |
The recovery after electroporation was performed in 1 mL of LB with 1mM of IPTG added in order to have the pBAD part have a high copy number | The recovery after electroporation was performed in 1 mL of LB with 1mM of IPTG added in order to have the pBAD part have a high copy number | ||
30 uL of 1M IPTG solution was added to a 25 mg/mL KAN plate and spread with sterile beads in order to keep the high copy plasmid in the pBAD part while plating | 30 uL of 1M IPTG solution was added to a 25 mg/mL KAN plate and spread with sterile beads in order to keep the high copy plasmid in the pBAD part while plating |
Revision as of 03:37, 31 July 2010