Team:Michigan/Oil Sands
From 2010.igem.org
(Difference between revisions)
(→7/28/2010) |
(→7/27/2010) |
||
Line 264: | Line 264: | ||
'''Miniprep of surface display''' | '''Miniprep of surface display''' | ||
- | At 9:00am a 5 mL culture of the newly transformed GFP part, the newly transformed linker part, the ompA part transformed previously and the INP from the UC davis team were started for a miniprep in LB with 100 mg/mL AMP. | + | At 9:00am a 5 mL culture of the newly transformed GFP part, the newly transformed linker part, the ompA part transformed previously and the INP from the UC davis team were started for a miniprep in LB with 100 mg/mL AMP. |
+ | |||
+ | The modified miniprep protocol was used listed on the protocol section of the wiki to get a higher DNA concentration | ||
At 9:00pm the GFP, linker and INP parts were miniprepped. The ompA culture had not grown out (b/c not enough -80C freezer stock was added) and a new culture was restarted at 11:00pm in 5 mL of LB with 100 mg/mL AMP. | At 9:00pm the GFP, linker and INP parts were miniprepped. The ompA culture had not grown out (b/c not enough -80C freezer stock was added) and a new culture was restarted at 11:00pm in 5 mL of LB with 100 mg/mL AMP. |
Revision as of 00:55, 31 July 2010