Team:SDU-Denmark/labnotes2

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(Annealing of the two mutated strands of FlhDC (FlhDCmut))
(Purification of pSB1A2 containing J13002)
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===Purification of pSB1A2 containing J13002===
===Purification of pSB1A2 containing J13002===
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<br>
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''Experiment done by:'' Pernille <br><br>
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''Date:'' the 19 of July <br><br>
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''Protocol:'' [https://2010.igem.org/Team:SDU-Denmark/protocols#Plasmid_miniprep_kit_.28Fermentas.29 MP1.1]<br><br>
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''Method:''  Miniprep, Nano drop and gel electrophoresis<br><br>
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''Notes:'' The pSB1A2 plasmid are a high-copy-plasmid and therefore 5mL ON culture are used for purification of the plasmid. <br><br>
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''Results:''<br><br> after the purification the concentration of nucleic acid concentration was measured on the Nano Drop. There was made four purification tubes. Only tube 1 and 3 was used for further experiments because the other contained a very low concentration of nucleic acid. The concentration in tube 1 and 3 was 25,72ng/uL and 25,76ng/uL.
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The purified DNA was loaded in a 1,5% agarose gel together with a 10.000bp long ladder (red).
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PCR was run on the DNA using the VR and VF2 primers wich ensures the only the brick part are amplificated. The pfu polymerase was used but unfortunately i did not get any results since i did not add any MgSO4 to the premix.
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--[[User:Pernm07|Pernm07]] 08:48, 22 July 2010 (UTC)
== Group: Photosensor ==
== Group: Photosensor ==

Revision as of 08:48, 22 July 2010