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Lab notes (7/19 - 7/25)

Contents 1 Lab notes (7/19 - 7/25) 1.1 Group: Flagella 1.1.1 Annealing of the two mutated strands of FlhDC 1.2 Group: Photosensor 1.3 Group: Retinal 1.3.1 Transformation of K081005 in pSB1A2 (constitutive promoter and RBS combined),R0011 in pSB1A2, pSB3C5 w. J04450 and pSB3T5 w. J04450 in Top 10 E.Coli

Group: Flagella

Annealing of the two mutated strands of FlhDC

Experiment done by: Sheila

Date: July 19th

Protocol: CP1.1

Method: PCR of the two mutated strands of the FlhDC operon

Notes: To samples were run at two different temperatures: 56,1˚C and 64,5˚C respectively.
Polymerase used: Pfu
Primers used: None, as the two strands are supposed to anneal to each other

Results:

Group: Photosensor

Group: Retinal

Transformation of K081005 in pSB1A2 (constitutive promoter and RBS combined),R0011 in pSB1A2, pSB3C5 w. J04450 and pSB3T5 w. J04450 in Top 10 E.Coli

Start date: 19/07 End date: 20/07
Methods: ON culture, making competent cells, transformation

Protocol:CC1.1 TR1.1

Experiment done by: Maria

Notes:ON colony was made of 110 ml lb medium inoculated with a top10 coloni.

Time	Optical  density
8:12	2.9
8:17	0.02
9:17	0.035
10:17	0.204
10:40	0.380
10:50	0.49

pSB1A2 w. R0011 and pSB1A2 w. K081005 was plated with 150uL on plates containing LA, LA + Amp, LA + Tetracycline, LA + Chloramphenicol and LA + Kanamycine.Upconcentration of these samples was not made. pSB3T5 w. J04450 and pSB3C5 w. J04450 was plated according to protocol

Results:

Analysis:
--Tipi 16:33, 19 July 2010 (UTC)

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