Team:SDU-Denmark/labnotes2

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(New page: {{:Team:SDU-Denmark/css2}} {{:Team:SDU-Denmark/navi2}} <div id="subnavi"> <div id="leftcolumn"> = Lab notes (7/19 - 7/25) = __TOC__ == Group: Flagella == <br> == Group: Photosensor == <...)
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== Group: Retinal ==
== Group: Retinal ==
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=== Transformation of K081005 in pSB1A2 (constitutive promoter and RBS combined),R0011 in pSB1A2, pSB3C5 w. J04450 and pSB3T5 w. J04450 in Top 10 E.Coli ===
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Start date: 19/07    End date: 20/07<br>
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''Methods:''  ON culture, making competent cells, transformation <br><br>
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''Protocol:''[https://2010.igem.org/Team:SDU-Denmark/protocols#CC1.1 CC1.1] [https://2010.igem.org/Team:SDU-Denmark/protocols#TR1.1 TR1.1] <br><br>
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''Experiment done by:'' Maria <br><br>
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''Notes:''ON colony was made of 110 ml lb medium inoculated with a top10 coloni.
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<html>
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<table style="text-align: left; width: 100px;" border="1"
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cellpadding="2" cellspacing="2">
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<tbody>
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<tr>
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<td style="vertical-align: top;">Time<br>
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</td>
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<td style="vertical-align: top;">Optical&nbsp; density<br>
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</td>
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</tr>
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<td style="vertical-align: top;">8:12<br>
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</td>
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<td style="vertical-align: top;">2.9<br>
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</tr>
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<td style="vertical-align: top;">8:17<br>
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</td>
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<td style="vertical-align: top;">0.02<br>
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</td>
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</tr>
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<tr>
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<td style="vertical-align: top;">9:17<br>
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</td>
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<td style="vertical-align: top;">0.035<br>
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</td>
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</tr>
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<td style="vertical-align: top;">10:17<br>
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</td>
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<td style="vertical-align: top;">0.204<br>
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</td>
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</tr>
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<td style="vertical-align: top;">10:40<br>
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</td>
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<td style="vertical-align: top;">0.380<br>
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</td>
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</tr>
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<td style="vertical-align: top;">10:50<br>
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</td>
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<td style="vertical-align: top;">0.49<br>
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pSB1A2 w. R0011 and pSB1A2 w. K081005 was plated with 150uL on plates containing LA, LA + Amp, LA + Tetracycline, LA + Chloramphenicol and LA + Kanamycine.Upconcentration of these samples was not made. pSB3T5 w. J04450 and pSB3C5 w. J04450 was plated according to protocol
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<br><br>
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''Results:''
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''Analysis:''<br>
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--[[User:Tipi|Tipi]] 16:33, 19 July 2010 (UTC)
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Revision as of 16:33, 19 July 2010

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