Team:Stanford/Notebook/Lab Work/Week 6

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== 8/2 Monday ==

===Greg's Notebook===

*Inoculated freezer stocks of most of our parts with Alex
*Began planning for promoter characterization project:
**Going to do 3A ligations of the form: promoter + superfolded GFP + Kan plasmid
**Process:
***Digest parts with at least 2 ug DNA
***PCR cleanup
***Diagnostic gel (remember to save uncut DNA for control)
***Ligate in PCR tubes
***Heat inactivate at 65 C for 20 min
*Performed first digestion (F2620 + sfGFP + pSB4k5)
{| align = "center" cellpadding = "5"
|      Part #      ||      Part amount      ||      BSA      ||      NEB (#, amount)      ||      Enzymes (1 uL each)      ||      Water 
|-
| F2620 || 10.8 || 3 || 2, 3 || E + S || 11.2
|-
| pSB4k5 || 20 || 3 || 3, 3 || E + P || 2
|-
| sfGFP || 11.76 || 3 || 2, 3 || X + P || 10.24
|}


== 8/3 Tuesday ==



== 8/4 Wednesday ==



== 8/5 Thursday ==



== 8/6 Friday ==

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