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From 2010.igem.org

Grew RosDE3 in 4 tubes (70 uL E. coli + 5mL LB + 5uL ChL) in 37 C

Shaker at 10:55am

Wait for the Log phase of the bacteria

O.D 0.483 blank

O.D 0.502 0.5mM

O.D 0.509 1mM

O.D 0.491 2.5mM

-Did 0, 2, 4 hr measurement on the agar plate