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Team:Yale/Our Project/Protocols/EtOH precipitate
From 2010.igem.org
our project
protocols
Standard DNA Precipitation Protocol
1. Add one-tenth volume of 3 M NaOAc, ph 5.2, to the DNA solution to precipitated.2. Add 2.5 volumes of cold 100% ethanol.
3. Place in -80°C freezer for 30 minutes of in an ethanol/dry ice bath for 5 minutes.
4. Spin at 15,000 rpm in microcentrifuge for 30 minutes at 4°C.
5. Rinse with cold 70% ethanol.
6. Spin at 15,000 rpm in microcentrifuge for 10 minutes at 4°C.
7. Remove supernatant, taking care of pellet, and dry fro 1 hour at 37°C.
8. Resuspend in appropriate volume of buffer.
