Construction Of K1, K2, K3

The samples inoculated previously were taken out and glecerol stocks were made.

The inoclum obtained from the overnight culture was used for plasmid extraction. The extracted plasmids were cut at specific restriction sites present in the biobrick sequence and run on the gel.`

The gel images of the bio-bricks with the band lengths were shown below in Fig1, FIg2, Fig3

K1-K2 Figure 1
K1-K2 Figure 2
K3 Figure 3

The gel was checked for correct fragment lengths to perform a second round f validation The extracted plasmids were digested with the correct set of enzymes as defined in the flow chart.

The concentration of plasmid were measured for all the samples.