"

From 2010.igem.org

Knightmare:

Plasmid extraction: AB-JK/N-TUV on PSB1C3 with determination by digesting.

Digestion: AB-JK/N-TUV on PSB1C3 with concentration determination.

Ligation: AB-JK-N-TUV.

Transfermation: AB-JK-N-TUV on PSB1C3 in old standard.