Team:UPO-Sevilla/Notebook/10 10

From 2010.igem.org

October, 10th

Assembly Team

Minipreps of UPO 11 samples and digestion. UPO 11 was confirmed by agarose gel.

Minipreps of 13+3 different inocula and digestion of them (2h, 37┬║C). Samples were run in a 0.8% agarose gel. UPO13+3/3T5 was confirmed.

Inocula of 1+2+7+3 different colonies were set up.

Transformation of UPO 12+2+16+3.

Digestion of 13+3 (to 3A and SA).

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