Aleksandra
Restriction digest with XbaI and SacI : 3µl miniprep + 2x1µl enzymes + 2µl B4 + 0.2µl BSA + 12.8µl water

• SacI-E-X-J23110/RBS/attP HK wt1-S-P-SacI
• SacI-E-X-J23110/RBS/attP HK wt2-S-P-SacI
• SacI-E-X-J23110/RBS/attP HK mut1-S-P-SacI
• SacI-E-X-J23110/RBS/attP HK mut2-S-P-SacI
• SacI-E-X-J23110/RBS/attP λ mut1-S-P-SacI
• SacI-E-X-J23110/RBS/attP λ mut2-S-P-SacI

Restriction digest with SpeI and SacI : 18µl miniprep + 2x3µl enzymes + 6µl B1 + 0.6µl BSA + 30µl water

• S-P-SacI-attP-SacI

</td></tr> <tr> <td style="background-color: #EEE"><a href="/wiki/Image:Igem-logo-150px.png" class="image" title="Igem-logo-150px.png"><img alt="" src="/images/7/7b/Igem-logo-150px.png" width="150" height="115" border="0" /></a>Population counter </td><td style="background-color: #F2F2F2" align="center"><img src="/images/9/94/Report.png" border="0" /> <a href="/wiki/IGEM:Paris_Liliane_Bettencourt/Notebook/Paris_2010" title="IGEM:Paris Liliane Bettencourt/Notebook/Paris 2010">Main project page</a>
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Léa
Glycerols and minipreps

• wRBS/LacZα
• sRBS/LacZα
• LuxI
• pLux
• attC/t/attC/mRFP
• Pm/mRBS/LuxR
• Pm/wRBS/LuxR
• wRBS/TetA(C)/LuxI
• mRBS/TetA(C)/LuxI
• sRBS/TetA(C)/LuxI
• Pm/wRBS/LuxR/attC/t/attC/mRFP
• pLux/LuxI (started with 3 colonies)

Nanodrop of the minipreps