# Team:Paris Liliane Bettencourt/Notebook/2010/08/06/

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Aleksandra
Transformation of the TURBO cells with yesterday's ligations (150µl per transformation)

• Pmed + mRFP
• Pst + mRFP
• Pmed/strRBS/LuxR + attC/term/attC/mRFP
• Pmed/medRBS/LuxR + attC/term/attC/mRFP
• Pmed/weakRBS/LuxR + attC/term/attC/mRFP
• Pstr/weakRBS/LuxR + attC/term/attC/mRFP
• Pstr/medRBS/LuxR + attC/term/attC/mRFP
• Pstr/strRBS/LuxR + attC/term/attC/mRFP
• Ps ctrl
• Pm ctrl
• Pm/weakRBS/LuxR ctrl
• Psstr/strRBS/LuxR ctrl

Raphaël
Minipreps
Elution in 60µl water

• Pm/weakRBS/LuxR
• Ps/weakRBS/LuxR
• Pm/medRBS/LuxR
• Ps/medRBS/LuxR
• Pm/strRBS/LuxR
• Ps/strRBS/LuxR

Elution in 30µl water

• weakRBS/TetA(C)
• medRBS/TetA(C)
• strRBS/TetA(C)
• weakRBS/LacZα
• strRBS/LacZα
• 11 minipreps of attC/term/attC/mRFP

Théotime
Overnight Culture in 12mL LB AMP.

• Pm/weakRBS/LuxR/attC/term/attC/mRFP (x3)
• Ps/weakRBS/LuxR/attC/term/attC/mRFP (x2)
• Pm/medRBS/LuxR/attC/term/attC/mRFP (x1)
• Ps/medRBS/LuxR/attC/term/attC/mRFP (x2)
• Pm/stdRBS/LuxR/attC/term/attC/mRFP (x3)
• Ps/stdRBS/LuxR/attC/term/attC/mRFP (x1)
• Ps/RFP (x4)