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From 2010.igem.org

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Annie Transformations of pucmod BB and pac BB. Plated overnight on ampicillin plates.

Tony and Matt Concentrations of GGFP BglII/Not I and pucBB BglII/Not I. Twenty five microliter reaction of GGFP BglII/NotI into pacmod BB BglII/Not I. Transformation of GGFP with 6/2 competent cells.