"
Korea U Seoul/4 September 2010
From 2010.igem.org
Guys! Check this out!
It's about tetR and Doxycycline
this one's about Doxycycline and its structure
tet: 
dox: 
- OOOOoooo check this out too!
https://2007.igem.org/Caltech/Project/Constructs
It's another wiki page and has some info on tetR-controlled activation
The figure below illustrates the expected behavior of the final construct system in E. coli bacteria. A constitutive promoter (both J23100 and J23116) continuously produces transcripts of the tet repressor gene, which when translated turns into tet repressor protein (tetR). The pTet promoter drives expression of the desired protein, N/Q/cro or YFP. In the absence of aTc, the tetR binds to pTet, greatly reducing the number of protein transcripts (and thus the protein concentration) in the cell. Adding aTc increases the protein concentration, as aTc binds to tetR and frees the pTet promoter to produce more transcripts. The pTet system allows us to reach intermediate expression levels, rather than switching between 'on' and 'off' states.
Construct Behavior: With no aTc in the system, tetR represses pTet promoter activity, resulting in a small number of the “protein” in the cell. With the addition of a saturating concentration of aTc, all the tetR protein is bound, leaving the pTet promoter free to produce the viral proteins that will lead viruses towards the lytic pathway.
- TetR structure
tetR-controlled activation regulation
When TetR binds to tetR operator, it makes the promoter attached to operator work
Binding of TetR to its operator site. A) tetR operator and contact regions. The tetR operator is a palindromic sequence. Horizontal bars show nucleotides contacted by each monomer of the TetR dimer. B) Interaction of TetR residues with specific nucleotides (arrows) and phosphate backbone (blue lines) in the operator region. The amino acids involved in DNA binding extend from residues 27 to 48. Contacts established with the operator were confirmed by footprint assays, by analysis of TetR mutants, and by crystallographic studies (29, 30, 159, 266, 366). C) Representation of each homodimer bound to the tet operator in a double-helix representation. (Adapted from Orth et al. [288] with permission of the publisher.)
--Needks 14:35, 4 September 2010 (UTC)
This is from cyworld club
회로를 이용해서 전사 레귤레이션하는 것들을 모아놓은거를 차자뜸!!!!><
위키 브레인스토밍에 올려놓음!
그리고 오늘일자로 tetR에 대한 내용도 올려놓음!!!
글구 생각해본게 몇개 있는데
이거 빠른시일내 우리 모두 생각해봐야할 거같아...
1. 중금속탐지 회로 완성
Cd Promoter/Cd gene----Zn Promoter/Zn gene----또뭐더라promoter/gene---kill promoter/kill gene-----
요러케 하는데 각 프로모터찾기...
kill promoter 같은 경우는 인듀서를 자연상태에 방치되었을때 가능한걸로...
뭐 UV라던가...? 라는 생각이 들어씀....
2. Toggle 스위치말야ㅑ....
그 우리가 논문에서 찾은 쌍방유전자 쓰던가...
아님 세개 유기적으로 돌아가는거 쓰던가 해야하는데...
일단 샘플이라도 만들어놓고~
1)일단 이 샘플로 가면 켜고 끄는것까지는 가능하나 그다음 못켠다
라고 쓰던가....
2)전기적으로 아세트알데히드같은것도 주입하니깐 그때 필요한 상황에 따라서 IPTG나 dox주입하게끔 컴퓨테이션 한다... 라던가....
3)아니면 우리가 tag를 붙여서 해결할 수 있는 방법이 있을 수도 있을 수도 있을거 같으니깐말야...
tag에 대해서 조금 더 알아보쟈규...
공부해보자!! --Needks 14:43, 4 September 2010 (UTC)
